2008 Addendum to the Instruction Manual 2.3, 08/2017)Īlanine aminotransferase (ALT) activity is determined by a kinetic method using a coupled enzyme reaction where the rate of reduced n icotinamide adenine dinucleotide (NADH) consumption is measured at 340 nm. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Unpublished Mayo method instruction manual: Siemens Nephelometer II, Siemens, Inc Version 2.3. The result is calculated by subtracting value of the final measurement from the initial measurement. An antigen-antibody complex is formed in the final measurement. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. The light is scattered onto the immuno-complexes that are present. A light beam is generated with a light-emitting diode, which is transmitted through the cuvette. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. ![]() If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume.Ī reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. ![]() In this method, the light scattered onto the antigen-antibody complexes is measured. Proprietary algorithm owned by BioPredictive Ratziu V, Charlotte F, Heurtier A, et al: Sampling variability of liver biopsy in nonalcoholic liver disease. Vilar-Gomez E, Chalasani N: Non-invasive assessment of non-alcoholic fatty liver disease: Clinical prediction rules and blood-based biomarkers. Munteanu M, Tiniakos D, Anstee Q, et al: Diagnostic performance of FibroTest, SteatoTest and ActiTest in patients with NAFLD using the SAF score as histological reference. Poynard T, Munteanu M, Charlotte F, et al: Impact of steatosis and inflammation definitions on the performance of NASH tests. Poynard T, Munteanu M, Charlotte F, et al: Diagnostic performance of a new noninvasive test for nonalcoholic steatohepatitis using a simplified histological reference. ![]() Munteanu M, Pais R, Peta V, et al: Long-term prognostic value of the FibroTest in patients with non-alcoholic fatty liver disease, compared to chronic hepatitis C, B, and alcoholic liver disease. Poynard T, Peta V, Munteanu M, et al: The diagnostic performance of a simplified blood test (SteatoTest-2) for the prediction of liver steatosis. BioPredictive: Technical Recommendations for FibroTest, FibroMax, and NASH-FibroTest assays, Bio Predictive. The Expert Panel on Integrated Guidelines for Cardiovascular Health and Risk Reduction in Children and Adolescents has set the following guidelines for lipids in children 2 to 17 years of age:įor SI unit Reference Values, see GLUCOSE FASTINGġ. The National Lipid Association and the National Cholesterol Education Program (NCEP) have set the following guidelines for lipids in adults 18 years old and older: A NashTest 2 score of 0.254 will round down to 0.25 and be staged N2.Ġ-6 days: Refer to for information on age-specific (postnatal hour of life) serum bilirubin values.ħ-14 days: or =18 years: or =14 years: 8-48 U/L For example, a NashTest 2 score of 0.245 will round up to 0.25 and be staged N0. *Boundary values can apply to 2 stages based on rounding.
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